enuMutRat on zeon

Estimating the probability of ENU-induced coding mutations

 This web site is designed to assist investigators employing ENU mutagenesis in the mouse. A conseqeunce of ENU's high rate of mutation is the possibility that a phenotype may be caused by a confounding unidentified mutation. This web site allows estimatation of the probability that one or more ENU induced coding mutations lies in a candidate region. Once the candidate region is specified, and the rate of mutation entered, this site will extract all coding DNA and apply the equations outlined in Keays et al 2006. These estimates are based on a Poisson model and assumption that: (1) the mutagenic process is independent and (2) that the rate of mutation is uniform.

Coding bases are identified using the UCSC Genome Annotations Known Gene tables. Overlapping CDS are reduced to a set of non-redundant coding seqs.

Mouse Genome Build 36 (Feb 2006) is used for this analysis. SNP locations are retrieved from the UCSC Genome Annotations (table snp126 in mm8)

Enter Chromosomal region:  Chromosome:  From:  To: 

OR

Between SNP  and SNP 

Mutation rate: 1 in  Mb

If you have any queries regarding the function of this web site please e-mail David Keays: keays (at) imp (dot) ac (dot) at

References:

Keays DA, Clark TG, Flint J. Estimating the number of coding mutations in genotypic- and phenotypic-driven N-ethyl-N-nitrosourea (ENU) screens.
Mamm Genome. 2006 Mar;17(3):230-8.

Keays DA, Clark TG, Campbell TG, Broxholme J, Valdar W. Estimating the number of coding mutations in genotypic and phenotypic driven N-ethyl-N-nitrosourea (ENU) screens: revisited.
Mamm Genome. 2007 Feb;18(2):123-4.

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Web interface by John Broxholme
Last modified: Fri Jul 10 10:51:59 BST 2009